3-Carboxyumbelliferylb-D-galactopyranoside,CUG-Carboxyumbelliferylb-D-galactoside

3-Carboxyumbelliferyl b-D-galactopyranoside , CUG; Carboxyumbelliferyl b-D-galactoside 3-Carboxyumbelliferyl b-D-galactopyranoside is a fluorescent probe that can be used to study protein synthesis in live cells. It binds to the active site of the enzyme peptidyl transferase, which catalyzes the covalent linkage of amino acids to form a polypeptide chain. 3CMBG is specific for the amino acid proline and is a substrate for peptidyl transferase. The fluorescence of 3CMBG increases when it is bound to peptidyl transferase, making it useful as a marker for protein synthesis. CUG (3-Carboxyumbelliferyl-?-D-galactopyranoside) is a fluorogenic substrate (?ex=386, ?em=445 nm, ?=32K). CAS Number 64664-99-9 Product Name 3-Carboxyumbelliferyl beta-D-galactopyranoside IUPAC Name 2-oxo-7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromene-3-carboxylic acid Molecular Formula C??H??O?? Molecular Weight 368.29 g/mol InChI InChI=1S/C16H16O10/c17-5-10-11(18)12(19)13(20)16(26-10)24-7-2-1-6-3-8(14(21)22)15(23)25-9(6)4-7/h1-4,10-13,16-20H,5H2,(H,21,22)/t10-,11+,12+,13-,16-/m1/s1 InChI Key HGMXXIAQZWTZLR-WUGLTUCPSA-N SMILES C1=CC2=C(C=C1OC3C(C(C(C(O3)CO)O)O)O)OC(=O)C(=C2)C(=O)O Canonical SMILES C1=CC2=C(C=C1OC3C(C(C(C(O3)CO)O)O)O)OC(=O)C(=C2)C(=O)O Isomeric SMILES C1=CC2=C(C=C1O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)OC(=O)C(=C2)C(=O)O