3-Indolyl-b-D-galactopyranoside

3-Indolyl b-D-galactopyranoside 3-Indolyl b-D-galactopyranoside (3IG) is a fluorescent probe that can be used to detect ?-gal expression in cells. 3IG is also known as ?-galactosidase. It is a protein that catalyzes the hydrolysis of the disaccharide lactose into glucose and galactose. 3IG is expressed in many tissues and cells, including those of the human prostate, ovary, colon, kidney, and lung. 3IG has been shown to bind specifically to polymerase chain reaction products of recombinant proteins and has been used for immunomagnetic detection of polymerase chain reaction products in tissues. 3IG also has phosphatase activity and can be used as a substrate for enzyme-linked immunosorbent assays (ELISA). CAS Number 126787-65-3 Product Name 3-Indolyl-b-D-galactopyranoside IUPAC Name (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(1H-indol-3-yloxy)oxane-3,4,5-triol Molecular Formula C14H17NO6 Molecular Weight 295.29 g/mol InChI InChI=1S/C14H17NO6/c16-6-10-11(17)12(18)13(19)14(21-10)20-9-5-15-8-4-2-1-3-7(8)9/h1-5,10-19H,6H2/t10-,11+,12+,13-,14-/m1/s1 InChI Key XVARCVCWNFACQC-MBJXGIAVSA-N SMILES C1=CC=C2C(=C1)C(=CN2)OC3C(C(C(C(O3)CO)O)O)O Canonical SMILES C1=CC=C2C(=C1)C(=CN2)OC3C(C(C(C(O3)CO)O)O)O Isomeric SMILES C1=CC=C2C(=C1)C(=CN2)O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O CAS No: 126787-65-3 Synonyms: 3-Indoxyl b-D-galactopyranoside MDL No: MFCD00083389 Chemical Formula: C14H17NO6 Molecular Weight: 295.29