4-Nitrophenyla-D-galactopyranoside

4-Nitrophenyl a-D-galactopyranoside The 4-Nitrophenyl-alpha-D-galactopyranoside is a high-quality chromogenic substrate specifically designed for the enzymatic assay of alpha-galactosidase. Upon enzyme catalysis, it yields a highly fluorescent 4-nitrophenol product, which allows easy and accurate measurement of enzyme activity in a variety of research applications, such as biochemical analysis, inhibitor screening, and enzyme kinetics studies. CAS No: 7493-95-0 Synonyms: PNP-a-Gal MDL No: MFCD00065050 Chemical Formula: C12H15NO8 Molecular Weight: 301.25 CAS Number 7493-95-0 Product Name 4-nitrophenyl-alpha-D-galactopyranoside IUPAC Name (2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol Molecular Formula C12H15NO8 Molecular Weight 301.25 g/mol InChI InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12+/m1/s1 InChI Key IFBHRQDFSNCLOZ-IIRVCBMXSA-N SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Synonyms 4-nitrophenyl-alpha-galactoside, 4-nitrophenylgalactoside, 4-nitrophenylgalactoside, (alpha-D)-isomer, p-nitrophenyl-alpha-D-galactopyranoside, p-nitrophenyl-alpha-galactoside, para-nitrophenyl-beta-D-galactoside, PNPG Canonical SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Isomeric SMILES C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O COA: name: 4-Nitrophenyl-?-D-galactopyranoside M.F.: C12H15O8 M.W.: 301.25 CAS: 7493-95-0 Items Standards Results Appearance White or slightly yellow crystal powder Complies Solubility Soluble in DMF and insoluble in ether Complies NMR and MS Should comply Complies Identification IR and HPLC Complies Specific rotation [?]20/D, (c=0.4, in water) +218? ~ +222? 220? Loss weight on dryness Max. 1% 0.5% Any impurity Max. 0.5% Complies Residue on ignition Max.1% 0.2% Heavy metals Max.20 ppm Complies TLC Should be one spot one spot Purity Min.98% 99.2% Assay by HPLC 98% ~ 102% 99.4%