p-Nitrophenyl2-acetamido-2-deoxy-alpha-D-glucopyranoside

4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside 4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chromogenic pNP substrate specifically designed for the analysis of N-acetylglucosaminidase activity. Upon enzyme action, the substrate releases 4-nitrophenol, a yellow compound that can be detected by spectrophotometric methods, providing a reliable and sensitive means of quantifying enzyme activity. This versatile substrate is widely used in biochemical studies, disease diagnostics, and the enzyme production industry. CAS Number 10139-02-3 Product Name 4'-Nitrophenyl-2-acetamido-2-deoxy-alpha-D-glucopyranoside IUPAC Name N-[(2R,3R,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-2-(4-nitrophenoxy)oxan-3-yl]acetamide Molecular Formula C14H18N2O8 Molecular Weight 342.3 g/mol InChI InChI=1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14+/m1/s1 InChI Key OMRLTNCLYHKQCK-KSTCHIGDSA-N SMILES CC(=O)NC1C(C(C(OC1OC2=CC=C(C=C2)[N+](=O)[O-])CO)O)O Synonyms GlcNAc1-?-PNP Canonical SMILES CC(=O)NC1C(C(C(OC1OC2=CC=C(C=C2)[N+](=O)[O-])CO)O)O Isomeric SMILES CC(=O)N[C@@H]1[C@H]([C@@H]([C@H](O[C@@H]1OC2=CC=C(C=C2)[N+](=O)[O-])CO)O)O CAS No: 10139-02-3 Synonyms: 4-Nitrophenyl N-acetyl-a-D-glucosamine MDL No: MFCD00006592 Chemical Formula: C14H18N2O8 Molecular Weight: 342.3 References: 1. Goldstein J, et al., J. Biol. Chem. 1987, 262, 4, 1596