Heparinase III, for EP Method

Heparinase III (heparin lyase III) cleaves heparan sulfate exclusively, and does not cleave unfractionated heparin or low molecular weight heparins. We provides heparinase III for determining 1,6-anhydro derivatives in heparin sodium. This enzyme has proven to meet the system suitability required by the European Pharmacopoeia method. System suitability required by the European Pharmacopoeia Method: – peak area ratio: maximum 1.15 for the peaks due to 1,6-anhydro ΔIS-IS and 1,6-anhydro ΔIS in the chromatogram obtained with reference solution (b); – maximum 0.02 for the peaks due to ΔIS and reduced ΔIS in the chromatogram obtained with reference solution (c); – resolution: minimum 1.5 between the peaks due to reduced ΔIA and 1,6-anhydro ΔIS in the chromatogram obtained with refer-ence solution (c); – the content of 1,6-anhydro derivatives in heparin sodium CRS is within 1.5 percent of the assigned content. Applications: Heparinase III is used for determining 1,6-anhydro derivatives in heparin sodiu