GDP-6-azido-fucose (GDP-6-N3-Fuc)

GDP-6-azido-fucose is an azide-bearing GDP-fucose analogue for fucosyltransferase and bioorthogonal labeling research. The azide provides a conjugation handle after an accepting enzyme transfers the modified sugar. Research context Chemoenzymatic preparation of GDP-azidofucose derivatives is documented in fucosyltransferase research. Select the enzyme and acceptor experimentally; this nucleotide donor is distinct from a cell-fed free fucose precursor. Natural L-fucose is a 6-deoxy sugar. Product selection and documentation Reference identifiers describe the labelled chemical form; they do not establish the counterion or hydration of a particular lot. Confirm the supplied form, assay, purity method and storage conditions on the lot certificate of analysis. Use the lot-specific formula weight when preparing molar solutions. Experimental planning Enzyme acceptance depends on the transferase, acceptor and reaction conditions. Validate the donor with your enzyme system; nucleotide sugars should not be assumed to enter cells simply because related monosaccharide precursors do.